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tris  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tris
    Tris, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tae/TAE+(10X)%2C+TRIS+%2B+acetate+%2B+EDTA/pm42286000-232-28-31
    Average 95 stars, based on 1 article reviews
    tris - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Downregulation of ClC-3 chloride channels in dorsal root ganglia neurons contributes to bone metastasis-induced pain.
    Article Snippet: .. For the Gel electrophoresis, 1× of TAE (Thermo 651 Fisher Scientific) diluted with ddH2O was used as a buffer system, 1% agarose gels in TAE buffer 652 containing 5 μl of 10,000 × GelRed (Beyotime Biotechnology) was used and poured into the 653 electrophoresis tank, allow the gels to dry, pull out the comb, and 5 μl of the samples was mixed 654 Jo urn al Pr e-p roo f 31 with 1 μl of 6 × DNA loading buffer (Takara, Berkeley, CA) and transferred to wells. .. The 655 Trans2K Plus II DNA Marker (TransGen Biotechnology) was also transferred to the sides of wells.

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Article Title: DNMT3a contributes to bone cancer pain by epigenetic silencing of Kcnq2/Kcnq3 in dorsal root ganglion neurons.
    Article Snippet: .. For the Gel electrophoresis, 1× of TAE (Thermo Fisher Scientific) diluted with ddH2O was used as a buffer system, 1% agarose gels in TAE buffer containing 5 μl of 10,000 × GelRed (Beyotime Biotechnology) was used and poured into the electrophoresis tank, allow the gels to dry, pull out the comb, and 5 μl of the samples was mixed with 1 μl of 6 × DNA loading buffer (Takara, Berkeley, CA) and transferred to wells. .. The Trans2K Plus II DNA Marker (TransGen Biotechnology) was also transferred to the sides of wells.

    Electrophoresis:

    Article Title: Downregulation of ClC-3 chloride channels in dorsal root ganglia neurons contributes to bone metastasis-induced pain.
    Article Snippet: .. For the Gel electrophoresis, 1× of TAE (Thermo 651 Fisher Scientific) diluted with ddH2O was used as a buffer system, 1% agarose gels in TAE buffer 652 containing 5 μl of 10,000 × GelRed (Beyotime Biotechnology) was used and poured into the 653 electrophoresis tank, allow the gels to dry, pull out the comb, and 5 μl of the samples was mixed 654 Jo urn al Pr e-p roo f 31 with 1 μl of 6 × DNA loading buffer (Takara, Berkeley, CA) and transferred to wells. .. The 655 Trans2K Plus II DNA Marker (TransGen Biotechnology) was also transferred to the sides of wells.

    Article Title: DNMT3a contributes to bone cancer pain by epigenetic silencing of Kcnq2/Kcnq3 in dorsal root ganglion neurons.
    Article Snippet: .. For the Gel electrophoresis, 1× of TAE (Thermo Fisher Scientific) diluted with ddH2O was used as a buffer system, 1% agarose gels in TAE buffer containing 5 μl of 10,000 × GelRed (Beyotime Biotechnology) was used and poured into the electrophoresis tank, allow the gels to dry, pull out the comb, and 5 μl of the samples was mixed with 1 μl of 6 × DNA loading buffer (Takara, Berkeley, CA) and transferred to wells. .. The Trans2K Plus II DNA Marker (TransGen Biotechnology) was also transferred to the sides of wells.

    Plasmid Preparation:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Expressing:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Agarose Gel Electrophoresis:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Article Title: Sampling and metabarcoding of arthropod environmental DNA traces from flowers
    Article Snippet: .. PCR conditions were as follows; initial denaturation of 1 cycle at 95 °C for 2 min followed by 35 cycles of denaturation at 98 °C for 20 s, annealing at 60 °C for 30 s and an extension at 72 °C for 30 s, and a subsequent final extension of 1 cycle at 72 °C for 2 min. After PCR amplification, 3 μl of the PCR product was electrophoresed on a 1% agarose gel (Nippon Gene, Japan) in TAE (40 mM Tris, 40 mM acetic acid, and 1 mM ethylenediaminetetraacetic acid) stained with SYBR Safe DNA gel stain (Invitrogen, USA) and observed under WSE-5400-UP Printgraph Classic (ATTO Corporation, Japan) mounted on a Safe Imager Blue Light Transilluminator (Invitrogen) to confirm if the eDNA extraction was a success. ..

    Gel Extraction:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Polymerase Chain Reaction:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Article Title: Sampling and metabarcoding of arthropod environmental DNA traces from flowers
    Article Snippet: .. PCR conditions were as follows; initial denaturation of 1 cycle at 95 °C for 2 min followed by 35 cycles of denaturation at 98 °C for 20 s, annealing at 60 °C for 30 s and an extension at 72 °C for 30 s, and a subsequent final extension of 1 cycle at 72 °C for 2 min. After PCR amplification, 3 μl of the PCR product was electrophoresed on a 1% agarose gel (Nippon Gene, Japan) in TAE (40 mM Tris, 40 mM acetic acid, and 1 mM ethylenediaminetetraacetic acid) stained with SYBR Safe DNA gel stain (Invitrogen, USA) and observed under WSE-5400-UP Printgraph Classic (ATTO Corporation, Japan) mounted on a Safe Imager Blue Light Transilluminator (Invitrogen) to confirm if the eDNA extraction was a success. ..

    Transferring:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Sterility:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Stripping Membranes:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    Imaging:

    Article Title: Directed Evolution in Escherichia coli for Novel Ligand‐Binding Regulators: Evolving a Progesterone‐Responsive Transcription Factor to Bind Cortisol
    Article Snippet: .. Q5 High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0492S) H 2 O, molecular biology‐grade (Life Technologies Corp., cat. no. AM9937) 10 μM forward primer, diluted in water (see Table ) 10 μM reverse primer, diluted in water (see Table ) pREG (regulator plasmid expressing SRTF1; Addgene, cat. no. 246680) DpnI (New England Biolabs, cat. no. R0176S) 6× gel loading dye, purple, no SDS (New England Biolabs, cat. no. B7025S) 0.8% agarose gel (see recipe) DNA ladder (New England Biolabs, cat. no. N0469S) 1× TAE (see recipe) Gel extraction kit (Thermo Scientific, cat. no. K0691) BsaI (New England Biolabs, cat. no. R3733S) T4 ligase buffer (New England Biolabs, cat. no. B0202S) T7 DNA ligase (New England Biolabs, cat. no. M0318S) PCR clean‐up kit (New England Biolabs, cat. no. T1030S) 20‐, 200‐, and 1000‐μl single‐channel pipettes (Rainin, or equivalent) 20‐, 200‐, and 1000‐μl pipette tips, filtered, sterile, (Rainin, or equivalent) PCR strip tubes (Axygen, cat. no. PCR‐0208‐AF‐C) Ice bucket and ice Thermocycler (Fisher Scientific, cat. no. 44‐840‐76, or equivalent) Mini centrifuge (LabForce, cat. no. 1154J27, or equivalent) Gel electrophoresis tank and accessories (Bio‐Rad Laboratories, cat. no. 1640302) Gel imager (ChemiDoc MP Imaging System, cat. no. 12003154) Razor blades (VWR, cat. no. 76457‐428) NanoDrop (Thermo Fisher Scientific, cat. no. ND‐2000C) ..

    other:

    Article Title: Kolteria novifilia, a novel planctomycetotal strain from the volcanic habitat of Panarea divides by unusual lateral budding
    Article Snippet: Amplicon bands (fragment size of ~300 bp) were cut out using sterile single-use scalpels and extracted using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany).

    Amplification:

    Article Title: Sampling and metabarcoding of arthropod environmental DNA traces from flowers
    Article Snippet: .. PCR conditions were as follows; initial denaturation of 1 cycle at 95 °C for 2 min followed by 35 cycles of denaturation at 98 °C for 20 s, annealing at 60 °C for 30 s and an extension at 72 °C for 30 s, and a subsequent final extension of 1 cycle at 72 °C for 2 min. After PCR amplification, 3 μl of the PCR product was electrophoresed on a 1% agarose gel (Nippon Gene, Japan) in TAE (40 mM Tris, 40 mM acetic acid, and 1 mM ethylenediaminetetraacetic acid) stained with SYBR Safe DNA gel stain (Invitrogen, USA) and observed under WSE-5400-UP Printgraph Classic (ATTO Corporation, Japan) mounted on a Safe Imager Blue Light Transilluminator (Invitrogen) to confirm if the eDNA extraction was a success. ..

    Staining:

    Article Title: Sampling and metabarcoding of arthropod environmental DNA traces from flowers
    Article Snippet: .. PCR conditions were as follows; initial denaturation of 1 cycle at 95 °C for 2 min followed by 35 cycles of denaturation at 98 °C for 20 s, annealing at 60 °C for 30 s and an extension at 72 °C for 30 s, and a subsequent final extension of 1 cycle at 72 °C for 2 min. After PCR amplification, 3 μl of the PCR product was electrophoresed on a 1% agarose gel (Nippon Gene, Japan) in TAE (40 mM Tris, 40 mM acetic acid, and 1 mM ethylenediaminetetraacetic acid) stained with SYBR Safe DNA gel stain (Invitrogen, USA) and observed under WSE-5400-UP Printgraph Classic (ATTO Corporation, Japan) mounted on a Safe Imager Blue Light Transilluminator (Invitrogen) to confirm if the eDNA extraction was a success. ..

    Extraction:

    Article Title: Sampling and metabarcoding of arthropod environmental DNA traces from flowers
    Article Snippet: .. PCR conditions were as follows; initial denaturation of 1 cycle at 95 °C for 2 min followed by 35 cycles of denaturation at 98 °C for 20 s, annealing at 60 °C for 30 s and an extension at 72 °C for 30 s, and a subsequent final extension of 1 cycle at 72 °C for 2 min. After PCR amplification, 3 μl of the PCR product was electrophoresed on a 1% agarose gel (Nippon Gene, Japan) in TAE (40 mM Tris, 40 mM acetic acid, and 1 mM ethylenediaminetetraacetic acid) stained with SYBR Safe DNA gel stain (Invitrogen, USA) and observed under WSE-5400-UP Printgraph Classic (ATTO Corporation, Japan) mounted on a Safe Imager Blue Light Transilluminator (Invitrogen) to confirm if the eDNA extraction was a success. ..



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